Authors:

Xie Tan

Abstract:

The aim of this study was to investigate the expression and clinical significance of the Tripartite motif-containing 74 gene in liver cancer.
Methods : Bioinformatics methods were used to analyze the gene expression of TRIM74 in liver cancer and normal liver tissues by using TCGA and GTEx databases.Wilcoxon test was used to analyze the differences in the expression of TRIM74 and to investigate the relationship between clinicopathological features.Kaplan-Meier method was used to examine the clinicopathological features of the correlation between TRIM74 expression and overall survival (OS).The expression
of TRIM74 protein and mRNA in liver cancer cell lines were detected by Western blotting and reverse transcription quantitative polymerase chain reaction (RT-qPCR), respectively.The overexpression of exogenous TRIM74 in HCC cells was used to observe the effects on the proliferation,migration and invasion of HCC cells.
Results : Compared with the non-cancerous liver tissues,the expression of TRIM74 in liver cancer tissues was significantly decreased, and the decreased expression was significantly correlated with the disease progression.Compared with HCC patients with high expression of TRIM74,the survival time of HCC patients with low expression of TRIM74 was significantly shortened.The protein and mRNA expression levels of TRIM74 in hepatoma cell lines were significantly decreased
compared with normal liver cell lines.Functional studies showed that overexpression of TRIM74 significantly reduced the proliferation,invasion and migration of HepG2 and Huh-7 cells.

Citation:

Xie Tan. Low expression of TRIM74 predicted poor prognosis and promoted the proliferation and migration of liver cancer cells. World Journal of Medical Oncology 2023